ABSTRACT
Objective To observe the effects of Zhuyunjiaonang on morphological change of the ovary, serous hormone and the expression of P450arom in PCO model rats. To explore its target and mechanism in treating PCO model rats. Methods Using INS and HCG to establish PCO model. The PCO rat models were devided into 4 groups randomly, which included control group, model group, Zhuyunjiaonang group and mefformin group. Zhuyunjiaonang group was given 3.0 g/(kg ? d) Zhuyunjiaonang, mefformin group was given 0.75 g/(kg ? d) metformin, control group and model group were given same volume distilled water 1 mL/100 g. The effects of Zhuyunjiaonang on ovaries and ovarial follicular development were observed by light microscope. The levels of serum gonadal hormone were detected by radioimmunoassay. The expression level of P450arom was detected with immtmohistochemical staining technique. Results Zhuyunjiaonang could increase the thickness of granular cell layer and decrease the thickness of theca cell layer, decrease the level of T and LH, and increase the level of FSH and E_2 in serum. Meanwhile the expression of P450arom in ovary were increased. Conclusion Zhuyunjiaonang could indirectly or directly affect the expression of the P450arom in ovary, it could regulate the endocrine function of PCO rat and promote the mature and ovulation of follicular.
ABSTRACT
Aim To observe the effect of metformin(MF)on inhibinB,activinA and follistatin in rat of polycystic ovary(PCO),analyzing the possible therapeutic mechanism and therapeutical effect from ovaries local cytokine.Methods The PCO rat models were set by insulin combined with human chorionic gonadotrophin(HCG).The levels of serum gonadal hormone and insulin(INS)were detected by radioimmunoassay.Using the sandwich ELISA technique the expressed level of serum inhibinB(INHB),activinA(ACTA)and follistatin(FS)was detected.Results Metformin could decrease the level of testosterone(T),luteotropic hormone(LH)and INS in rat serum,but it increased the level of ACTA in rat serum and the rate of ACTA/INHB.There was negative correlation between INS and ACTA,and positive correlation between INS and INHB.Conclusion MF can regulate the expression of ovaries local cytokines such as INHB,ACTA and FS.